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Strangeways, Here We Come Strangeways, Here We Come is the band’s final studio album.

Well received, the album is included on Rolling Stone’s “500 Greatest Albums of All Time,” Pitchfork’s “Best Albums of the 1980s” and Slant’s “Best Albums of the 1980s.” It includes the standouts “Bigmouth Strikes Again,” “The Boy With The Thorn In His Side,” “There Is A Light That Never Goes Out” and the title-track. It reached #2 in the United States and spent twenty-two weeks on the U.K. This study demonstrates the potential for differential bias in bacterial community profiles resulting from the choice of sequencing platform alone.While building on their trademark guitar-pop, the album includes the standouts “The Headmaster Ritual” and “That Joke Isn’t Funny Anymore.” Meat Is Murder is included on Rolling Stone’s “500 Greatest Albums of All Time.” The Queen Is Dead The Queen Is Dead is The Smiths’ Gold-certified masterpiece. Disparities could be attributed to the failure to generate full-length reads for particular organisms on the Ion Torrent platform, organism-dependent differences in sequence error rates affecting classification of certain species, or some combination of these factors. Results of bacterial community profiling performed on the mock community and a collection of 18 human-derived microbiological specimens were generally in good agreement for both platforms however, in some cases, results differed significantly.

We found that these sequencing artifacts could be minimized by using bidirectional amplicon sequencing and an optimized flow order on the Ion Torrent platform. Read truncation was dependent on both the directionality of sequencing and the target species, resulting in organism-specific biases in community profiles. We observed comparatively higher error rates with the Ion Torrent platform and report a pattern of premature sequence truncation specific to semiconductor sequencing. We benchmarked performance by using a 20-organism mock bacterial community and a collection of primary human specimens. Here we compare the performances of two common “benchtop” sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16S rRNA (V1-V2) amplicon sequencing. High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity.